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Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone

Article DOI: 10.3791/54929-v • 09:24 min • December 15th, 2016
December 15th, 2016 •
1, 1, 1
1Department of Anatomy, Korea University College of Medicine

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Establishing culture systems for the expansion of adult neural stem cells (aNSCs) allows for the examination and application of aNSCs for therapy. The subcallosal zone (SCZ) has recently been recognized as a novel neuroblast-forming region in adult mice. Here, methods for the isolation, expansion, and differentiation of SCZ-aNSCs are described.

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Isolation Culture Adult Neural Stem Cells Mouse Subcallosal Zone Nonadherent Culture System Adherent Culture System Brain Lesions Technique Brain Matrix Razor Blade Forceps PBS Dissection Microscope Surgical Tools F-12 DMEM Medium B-27 Supplement N-2 Supplement Penicillin-streptomycin Digestion Buffer Poly-L-Ornithine Laminin Small Plates Maintenance Monolayer Cover Slips Immunostaining
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