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En Face Preparazione delle navi sanguigne del mouse
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En Face Preparazione delle navi sanguigne del mouse

Article DOI: 10.3791/55460-v • 10:01 min • May 19th, 2017
May 19th, 2017 •
1, 1, 2, 1
1Department of Cardiology, Division of Internal Medicine, University of Texas MD Anderson Cancer Center, 2Department of Radiation Oncology, University of Texas MD Anderson Cancer Center

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Viene descritta una procedura per preparare preparazioni in faccia della arteria e dell'arto carotide del topo. Tali preparati, quando vengono colorati immunofluorescenti con anticorpi specifici, ci permettono di studiare la localizzazione delle proteine ​​e l'identificazione dei tipi di cellule nell'intera parete vascolare tramite la microscopia confocale.

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Protocollo di base Numero 123 Aorta arteria carotidea cellule endoteliali en faccia preparazione immunofluorescenza
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