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Beoordeling van Sarcoplasmic Reticulum Calcium Reserve en intracellulaire diastolische Calcium verwijdering in geïsoleerde ventriculaire Cardiomyocytes
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Beoordeling van Sarcoplasmic Reticulum Calcium Reserve en intracellulaire diastolische Calcium verwijdering in geïsoleerde ventriculaire Cardiomyocytes

Article DOI: 10.3791/55797-v • 11:00 min • September 18th, 2017
September 18th, 2017 •
*1, *2, 1, 1, 3, 1, 1
1Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 2Experimental Research Center, China Academy of Chinese Medical Sciences, 3Department of Cardiology, The Third Hospital of Hangzhou City
* These authors contributed equally

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Recycling van intracellulair calcium speelt een cruciale rol in de regulering van de systolische en diastolische functie in cardiomyocytes. Hier beschrijven we een protocol om te evalueren van sarcoplasmic reticulum Ca2 + reserve en diastolische calcium functie voor het verwijderen in cardiomyocytes door een calcium imaging systeem.

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Celbiologie kwestie 127 Sarcoplasmic reticulum calcium-homeostase calcium reserve intracellulair calcium verwijdering SERCA cardiomyocytes
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