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Journal / Developmental Biology / Bioengineering af humaniserede knoglemarv mikro…
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Developmental Biology

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JoVE Journal Developmental Biology
Bioengineering af humaniserede knoglemarv mikro miljøer i mus og deres visualisering ved Live Imaging
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Bioengineering af humaniserede knoglemarv mikro miljøer i mus og deres visualisering ved Live Imaging

Article DOI: 10.3791/55914-v • 10:03 min • August 1st, 2017
August 1st, 2017 •
*1, *1, 1, 1, 1
1Haematopoietic Stem Cell Laboratory, The Francis Crick Institute
* These authors contributed equally

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En metode til at skabe og levende billede forskellige humaniserede knoglemarv niske i mus er præsenteret. Baseret på den støttende niche, der er skabt af humane mesenkymceller, frembringer tilsætningen af ​​humane endotelceller dannelsen af ​​humane fartøjer, mens tilsætningen af ​​rhBMP-2 inducerer dannelsen af ​​humant mu kimært modent knoglevæv.

Tags

Udviklingsbiologi udgave 126 levende billeddannelse tofotonmikroskopi knoglemarv stroma mikromiljø humaniseret niche humane hæmatopoietiske stamceller human leukæmi vaskulatur endotelceller mesenkymale stromale celler BMP2
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