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Journal / Biochemistry / Obtaining High-Quality Transcriptome Data from…
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Biochemistry

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JoVE Journal Biochemistry
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
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Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Article DOI: 10.3791/60602-v • 07:18 min • May 21st, 2020
May 21st, 2020 •
1, 2, 3, 2
1Department of Chemistry and Biotechnology, Swinburne University of Technology, 2Department of Molecular Genetics, Heterosis Research Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 3Grain Quality and Nutrition Center, Applied Functional Genomics Cluster, International Rice Research Institute

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Summary
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A method for transcriptome profiling of cereals is presented. The microarray-based gene expression profiling starts with the isolation of high-quality total RNA from cereal grains and continues with the generation of cDNA. After cRNA labelling and microarray hybridization, recommendations are given for signal detection and quality control.

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High-quality Transcriptome Data Cereal Seeds Modified Method Gene Expression Profiling Microarray Hybridization High Throughput Sequencing Cost-effective Analysis Dr. Christiane Seiler Gene Expression Hybridization Kit Blocking Agent Fragmentation Mix
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