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Biology

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Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
 

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Article DOI: 10.3791/61517-v 08:33 min August 5th, 2020
August 5th, 2020

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Summary

Presented here is a protocol for laser-capture microdissection (LCM) of plant tissues. LCM is a microscopic technique for isolating areas of tissue in a contamination-free manner. The procedure includes tissue fixation, paraffin embedding, sectioning, LCM and RNA extraction. RNA is used in the downstream tissue-specific, temporally resolved analysis of transcriptomes.

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Laser-Capture Microdissection RNA-Sequencing Spatial Gene Expression Analysis Temporal Gene Expression Analysis Tissue-specific Gene Expression Analysis Plants Laser Technique Visualization Of Cells Isolation Of Cells Contact-free Isolation Sample Preparation Tissue Fixation Embedding Barley Seed Fixative Vacuum Infiltration Tissue Processing
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