Articles by Margaret L. Warner in JoVE

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

JoVE 2649 6/03/2011

Taylor R. Fore¹, Audrey A. Ojwang¹, Margaret L. Warner¹, Xinyun Peng¹, Rudolf A. Bohm^1,2, William P. Welch¹, Lindsey K. Goodnight¹, Hong Bao¹, Bing Zhang¹

¹Department of Zoology, University of Oklahoma - Norman, ²Department of Biology, Brandeis University

We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping.