Articles by Paul Lueth in JoVE

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

JoVE 3884 6/08/2012

Yashdeep Phanse¹, Amanda E. Ramer-Tait¹, Sherree L. Friend², Brenda Carrillo-Conde³, Paul Lueth¹, Carrie J. Oster¹, Gregory J. Phillips¹, Balaji Narasimhan³, Michael J. Wannemuehler¹, Bryan H. Bellaire¹

¹Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, ²Amnis Corporation, ³Department of Chemical and Biological Engineering, Iowa State University

In this article, we describe a method utilizing multi-spectral imaging flow cytometry to quantify the internalization of polyanhydride nanoparticles or bacteria by RAW 264.7 cells.