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In JoVE (5)
- Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
- Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice
- Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay
- Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat
- Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion
Other Publications (17)
- The Pediatric Infectious Disease Journal
- BMC Neurology
- Journal of Neurosurgery
- Journal of Neurosurgery
- Journal of Neurosurgery
- Journal of Neurosurgery. Pediatrics
- Journal of Neurosurgery. Pediatrics
- Pediatric Neurosurgery
- Journal of Neurosurgery. Pediatrics
- Pediatric Neurosurgery
- Neurosurgical Review
- Neurosurgical Review
- Journal of Neurointerventional Surgery
- Pain Practice : the Official Journal of World Institute of Pain
- World Neurosurgery
- Anatomy & Cell Biology
- AAPS PharmSciTech
Articles by Saeed Ansari in JoVE
JoVE Neuroscience
Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
1Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran, 2Department of Neurosurgery, The University of Florida
This video protocol demonstrates the application of the neurosphere assay for the isolation and expansion of neural stem cells from the ganglionic eminences of embryonic day 14-mouse brain.
JoVE Clinical and Translational Medicine
Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice
1Department of Neurosurgery, University of Florida, 2Department of Anatomical Sciences, Shiraz University of Medical Sciences
The intraluminal middle cerebral artery occlusion (MCAO) model is the most frequent used model among experimental ischemic stroke models. Here we will demonstrate the entire model in detail with the guide of Laser Doppler flowmetry, and its representative results.
JoVE Neuroscience
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay
1Department of Neurosurgery, University of Florida, 2Department of Anatomical Sciences, Shiraz University of Medical Sciences
This video protocol demonstrates the isolation and expansion of stem like cells from surgically resected human glioblastoma mutliforme (GBM) tumor tissue using the neurosphere assay culture method.
JoVE Clinical and Translational Medicine
Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat
1Department of Neurosurgery, University of Florida, 2Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran, 3Department of Physiology and Functional Genomics, University of Florida, 4Department of Neurology, University of Florida
Several animal models of cerebral ischemia have been developed to simulate the human condition of stroke. This protocol describes the endothelin-1 (ET-1) induced middle cerebral artery occlusion (MCAO) model for ischemic stroke in rats. In addition, important considerations, advantages, and shortcomings of this model are discussed.
JoVE Clinical and Translational Medicine
Utilizing a Cranial Window to Visualize the Middle Cerebral Artery During Endothelin-1 Induced Middle Cerebral Artery Occlusion
1Department of Physiology and Functional Genomics, University of Florida, 2Department of Neurosurgery, McKnight Brain Institute, University of Florida, 3Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
This article describes a method for visualizing rat cerebral arteries through a cranial window using temporal craniectomy in order to view proximal portions of the middle cerebral artery (Figure 1). This versatile method can be combined with various techniques of drug delivery to measure cerebral artery reactivity in vivo.
Other articles by Saeed Ansari on PubMed
Are the Distributions of Variations of Circle of Willis Different in Different Populations? - Results of an Anatomical Study and Review of Literature
Previous studies have proposed correlation between variants of the cerebral arterial circle (also known as circle of Willis) and some cerebrovascular diseases. Differences in the incidence of these diseases in different populations have also been investigated. The study of variations in the anatomy of the cerebral arterial circle may partially explain differences in the incidence of some of the cerebrovascular diseases in different ethnic or racial groups. While many studies have investigated the variations in the anatomy of each segment of the cerebral arterial circle, few have addressed the variants of the cerebral arterial circle as a whole. Similarly, the frequency of occurrence of such variants in different ethnic or racial groups has not been compared.
Ruptured Occipitocervical Teratoma Mimicking an Upper Cervical Myelomeningocele. Case Report
Occipitocervical teratoma is an extremely rare condition, and only a few cases have been reported. The authors report on a neonate who harbored a ruptured posterior midline occipitocervical lesion that mimicked an upper cervical myelomeningocele, although the pathological findings were diagnostic of teratoma.
Possible Genetic Correlation of an Occipital Dermal Sinus in a Mother and Son. Case Report
Occipital dermal sinuses (ODSs) are congenital lesions located in the midline and characterized by a cutaneous pit or dimple. The intracranial extension as well as the associated symptoms are variable. To date, a familial occurrence of these lesions has not been reported. In this paper the authors report on a 2-year-old boy with an ODS and intracranial hypertension. The boy's mother had a similar lesion but did not have any complaints. Following their experience with this case and a literature review, the authors suggest that there may be a genetic basis in certain instances of ODS.
Split Cord Malformation Associated with Myelomeningocele
The coexistence of split cord malformations (SCMs) and myelomeningoceles (MMCs) can be misdiagnosed or ignored and can cause spinal cord tethering. The authors investigated cases involving the coexistence of SCMs and MMCs in patients from the Children's Hospital Medical Center in Tehran.
Spina Bifida Occulta: is It a Predictor of Underlying Spinal Cord Abnormality in Patients with Lower Urinary Tract Dysfunction?
The purpose of this study was to evaluate the importance of spina bifida occulta in radiographs of children with lower urinary tract or bowel dysfunction.
Breastfeeding: a Potential Protective Factor Against Ventriculoperitoneal Shunt Infection in Young Infants
Previous studies have shown nutritional benefits of breastfeeding for a child's health, especially for protection against infection. Protective factors in human milk locally and systemically prevent infections in the gastrointestinal as well as upper and lower respiratory tracts. It remains unclear whether breastfeeding protects infants against ventriculoperitoneal (VP) shunt infection.
The Cerebral Arterial Circle (circulus Arteriosus Cerebri): an Anatomical Study in Fetus and Infant Samples
Many studies have investigated the variations in the anatomy of each segment of the cerebral arterial circle while a few have addressed the variations of the cerebral arterial circle as a whole.
Risk Factors Associated with Occipital Encephalocele: a Case-control Study. Clinical Article
An encephalocele is characterized by congenital herniation of the brain tissue and/or meninges through a skull defect. The underlying cause is complex and not fully understood, but environmental agents are suspected. The authors aimed to determine the known risk factors for encephaloceles.
Triple Lumbar Dermal Sinuses: Unusual Presentation of a Typically Solitary Midline Lesion
Congenital dermal sinus is a type of closed spinal dysraphism caused by incomplete disjunction. The lesion occurs everywhere along neuraxis predominantly as a solitary midline sinus in the lumbosacral area.
Recanalization Therapy for Acute Ischemic Stroke, Part 2: Mechanical Intra-arterial Technologies
Stroke therapy has been revolutionized in the past two decades with the widespread implementation of chemical thrombolysis for acute stroke. However, chemical thrombolysis continues to be limited in its efficacy secondary to relatively short time windows and a high associated risk of hemorrhage. In an attempt to minimize hemorrhagic complications and extend the available therapeutic window, mechanical devices designed specifically for thrombus removal, clot obliteration, and arterial revascularization have experienced a recent surge in development and utilization. As such, chemical thrombolytics now represent only one of many options in acute stroke therapy. These new mechanical devices have extended the potential treatment window and now provide alternatives to patients who do not respond to conventional intravenous thrombolysis. This review will discuss the development of these devices, supporting literature, and the individual strengths that each engenders towards a life-saving therapy for stroke.
Recanalization Therapy for Acute Ischemic Stroke, Part 1: Surgical Embolectomy and Chemical Thrombolysis
Cerebral revascularization approaches for acute ischemic stroke greatly expanded during the past decade. Many new revascularization strategies are currently being assessed, while others continue to gain in popularity, offering hope to those with an otherwise refractory disease. We discuss historical and current progress toward successful recanalization, as well as the efforts being made to develop a safe and efficacious method of revascularization in the treatment of acute ischemic stroke.
Impact of Intracranial Self-expanding Stents in the Treatment of Acute Ischemic Stroke: Efficacy and Limitations
In recent years, endovascular self-expanding stents have become a viable option for neurointerventionalists treating acute ischemic stroke. Timely intervention for stroke could mean the difference between complete recovery and significant morbidity or death. Since 2006, a handful of clinical trials have demonstrated the potential of self-expanding stents in dependably achieving quick revascularization. These devices are expanding in utilization as new designs offer greater procedural flexibility and better clinical results. We discuss these clinical investigations, focusing on the procedural capabilities and limitations of each self-expanding stent design.
The Peridural Membrane of the Spinal Canal: A Critical Review
There exists substantial evidence that a peridural membrane (PM) is present in the spinal canal of humans and, like the pleura and peritoneum, has one or more physiologic functions. Innervation of the PM suggests that it may become a source of pain if injured. Although debated, the physiology of this structure has important implications with respect to neuraxial distribution of drugs and for back and radiating pain. This review, separated into embryological, anatomic, and physiologic discussions, provides an in-depth summary of the observations of this connective tissue. The discrepancies between accounts are highlighted within each section. Focused research to clearly elucidate the true nature of the PM, especially as related to neuraxial distribution of drugs and back and radiating pain, is warranted.▪
Intracranial Stents for Treatment of Acute Ischemic Stroke: Evolution and Current Status
Intravascular stents have been applied to treat a variety of pathophysiologic conditions. With advances in stent design and delivery, stenting has become a viable treatment option in neurovascular disease. Recently, intracranial arterial stenting has received increasing interest as a modality to rapidly and effectively recanalize affected vessels in the setting of acute ischemic stroke.
A Simple Technique for Morphological Measurement of Cerebral Arterial Circle Variations Using Public Domain Software (Osiris)
This article describes a straightforward method to measure the dimensions and identify morphological variations in the cerebral arterial circle using the general-purpose software program Osiris. This user-friendly and portable program displays, manipulates, and analyzes medical digital images, and it has the capability to determine morphometric properties of selected blood vessels (or other anatomical structures) in humans and animals. To ascertain morphometric variations in the cerebral arterial circle, 132 brains of recently deceased fetuses, infants, and adults were dissected. The dissection procedure was first digitized, and then the dimensions were measured with Osiris software. Measurements of each vessel's length and external diameters were used to identify and classify morphological variations in the cerebral arterial circle. The most commonly observed anatomical variations were uni- and bilateral hypoplasia of the posterior communicating artery. This study demonstrates that public domain software can be used to measure and classify cerebral arterial circle vessels. This method could be extended to examine other anatomical regions or to study other animals. Additionally, knowledge of variations within the circle could be applied clinically to enhance diagnostic and treatment specificity.
Anti-inflammatory Effect of Seeds and Callus of Nigella Sativa L. Extracts on Mix Glial Cells with Regard to Their Thymoquinone Content
Anti-inflammatory effect of the alcoholic extracts of N. sativa seeds and its callus on mix glial cells of rat with regard to their thymoquinone (TQ) content was investigated. Callus induction was achieved for explants of young leaf, stem, petiole, and root of N. sativa on solid Murashige and Skoog (MS) medium containing 2,4-D (1 mg/l) and kinetin (2.15 mg/l). TQ content of the alcoholic extracts was measured by HPLC. Total phenols were determined using Folin-Ciocalteu method and antioxidant power was estimated using FRAP tests. The mix glial cells, inflamed by lipopolysaccharide, were subjected to anti-inflammatory studies in the presence of various amounts of TQ and the alcoholic extracts. Viability of the cells and nitric oxide production were measured by MTT and Griess reagent, respectively. The leaf callus obtained the highest growth rate (115.4 mg/day) on MS medium containing 2,4-D (0.22 mg/l) and kinetin (2.15 mg/l). Analyses confirmed that TQ content of the callus of leaf was 12 times higher than that measured in the seeds extract. However, it decreased as the calli aged. Decrease in the TQ content of the callus was accompanied with an increase in its phenolic content and antioxidant ability. Studies on the inflamed rat mix glial cells revealed significant reduction in the nitric oxide production in the presence of 0.2 to 1.6 mg/ml of callus extract and 1.25 to 20 μl/ml of the seed extracts. However, the extent of the effects is modified assumingly due to the presence of the other existing substances in the extracts.
