Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove

Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells

November 10th, 2023
*1, *1, 1, 2, 1
1Manipal Institute of Regenerative Medicine, Bengaluru, Manipal Academy of Higher Education, Manipal, 2HIV-AIDS Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR)
* These authors contributed equally

Summary
Automatic Translation

This protocol describes the use of fluorescence-activated cell sorting of human mesenchymal stem cells using the single-cell sorting method. Specifically, the use of single-cell sorting can achieve 99% purity of the immunophenotyped cells from a heterogeneous population when combined with a multiparametric flow cytometry-based approach.

Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter