Here, we introduce a stability evaluation method based on multiple light scattering technology to evaluate the stability of traditional Chinese medicine extracts.
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Method Article
Using Multiple Light Scattering to Examine the Stability of Phyllanthus emblica L. Extracts Obtained with Different Extraction Methods
* These authors contributed equally
In This Article
Erratum Notice
Important: There has been an erratum issued for this article. View Erratum Notice
Summary
Abstract
The extraction intermediate of traditional Chinese medicine is the key intermediate in the preparation process, and its stability has an important impact on the effectiveness and quality of the final product. However, existing stability evaluation methods are often time-consuming and labor-intensive, requiring long-term observation and the operation of complex equipment (such as high-performance liquid chromatography), and it is difficult to obtain more physical information about the instability of the system. Therefore, there is an urgent need to establish a fast and accurate stability analysis technology for traditional Chinese medicine. Multiple light scattering is a cutting-edge analytical method that can accurately and rapidly evaluate the stability of traditional Chinese medicines in an environment-friendly manner without changing the nature or state of the sample or using organic reagents.
In this work, using the precise scanning data of multiple light scattering, the present protocol rapidly acquired the variation curves for layer thickness, particle migration speed, and average particle size over time. This enabled the precise identification of the mechanism and crucial characteristics causing the system's instability in its early stages. Of note, the research period for the extraction process can be considerably shortened by the detailed quantification of the system stability, which also allows for a quick, accurate, and in-depth analysis of the effects of various extraction processes on the stability of Phyllanthus emblica L.
Introduction
In the manufacturing of traditional Chinese medicine (TCM), the stability of the TCM extraction intermediates and related liquid preparations has always been the focus of inspection1. The clinical efficacy of medicinal products, especially with polyphenols as the primary active ingredient, suffers due to significant stability issues2,3. Sanajon oral liquid and Nuodikang oral liquid are examples of typical cases of this issue4. Therefore, it is crucial to learn how to use efficient tools to rapidly and accurately evaluate and optimize the stability of liquid inter....
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Protocol
1. Extract preparation
- Accurately weigh an appropriate amount of PE, and add 10x (weight) of deionized water for reflux extraction.
- Set five samples for reflux extraction for 0 h (E1), 0.5 h (E2), 1 h (E3), 1.5 h (E4), and 2 h (E5) after weighing.
- After extraction, cool the samples to room temperature, and weigh to make up for the lost weight to ensure consistency with the pre-extraction weights.
- Centrifuge the samples at 8,581 × g for 10 min to ensure the removal of insoluble material and herbal residues from the sample solution.
- Use a pipette to add 20 mL of sample solution into the s....
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Results
Figure 1 shows the principle of multiple light measurement and the meaning of the collected results. In the MLS spectra results (Figure 2), the abscissa was the height of the sample cell, and the ordinate was the transmission (T%) and backscattering (BS%) intensity. By calculating the MLS spectra results, the system can obtain the changes in the key physical parameters of the sample during the measurement period, including the delta transmissio.......
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Discussion
The rapid and accurate assessment of TCM stability has always been a focus of TCM research. To provide more useful information for directing the improvement of the extraction process, this study analyzed the stability and instability mechanisms of a sample using a near-infrared non-destructive technology.
In this protocol, the important stability parameters are calculated based on accurate MLS scan data. MLS scans can collect the transmission (T%) and backscattering (BS%) of .......
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Disclosures
The authors have no conflicts of interest to disclose.
Acknowledgements
This study was supported by grants from the National Natural Science Foundation of China (81973493); National Interdisciplinary Innovation Team of Traditional Chinese Medicine (ZYYCXTD-D-202209); Sanajon Pharmaceutical Group Chengdu University of TCM production, study, and Research Joint Laboratory Project (2019-YF04-00086-JH); and Sichuan Province Science and Technology Plan Funded Project (2021YFN0100). The authors thank the Innovative Institute of Chinese Medicine and Pharmacy of Chengdu University of TCM for its technical support in the mass spectrometry work.
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Materials
| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| Adjustable electric heating jacket | Beijing Kewei Yongxing Instrument Co., Ltd | MH-1000 | www.keweiyq.com |
| Analytical balance(1/10000) | Sartorious, Germany | BSA224S | www.sartorius.com.cn |
| CNC ultrasonic instrument | Kunshan Ultrasonic Instrument Co., Ltd | KQ-500DE | www.ks-csyq.com |
| GL-16 high-speed centrifuge | Sichuan Shuke Instrument Co., Ltd | 18091403 | www.sklxj.com |
| Phyllanthus emblica L. | Hehuachi medicinal materials market | YJL2004 | Produced in Yunnan |
| Turbisoft Lab multiple light scattering instrument | French Formulaction Company | Turbisoft Lab 2.3.1.125 Fanalyser 1.3.5 | www.formulaction.com |
| UPR-II-5T ultra-pure water device | Sichuan ULUPURE Ultrapure Technology Co., Ltd | Z16030559 | www.ccdup.com |
References
- Huang, H. -Z., et al. Exploration on the approaches of diverse sedimentations in polyphenol solutions: An integrated chain of evidence based on the physical phase, chemical profile, and sediment elements. Frontiers in Pharmacology. 10, 1060(2019).
- Ran, F., et al.
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Reprints and Permissions
Erratum
Formal Correction: Erratum: Using Multiple Light Scattering to Examine the Stability of Phyllanthus emblica L. Extracts Obtained with Different Extraction Methods
Posted by JoVE Editors on 8/04/2023. Citeable Link.
An erratum was issued for: Using Multiple Light Scattering to Examine the Stability of Phyllanthus emblica L. Extracts Obtained with Different Extraction Methods. The Authors section was updated from:
Haozhou Huang1
Mengqi Li2
Chuanhong Luo3
Sanhu Fan4
Taigang Mo4
Li Han3
Dingkun Zhang3
Junzhi Lin5
1Innovative Institute of Chinese Medicine and Pharmacy/Academy for Interdiscipline, Chengdu University of Traditional Chinese Medicine
2Sichuan Nursing Vocational College
3School of Pharmacy/School of Modern Chinese Medicine Industry, State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China
4Sanajon Pharmaceutical Group
5TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine
to
Haozhou Huang1,2
Mengqi Li3
Chuanhong Luo4
Sanhu Fan5
Taigang Mo5
Li Han4
Dingkun Zhang4
Junzhi Lin6
1State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy/Academy for Interdiscipline, Chengdu University of Traditional Chinese Medicine
2Meishan Hospital of Chengdu University of Traditional Chinese Medicine
3Sichuan Nursing Vocational College
4State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine
5Sanajon Pharmaceutical Group
6TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine
