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Bioengineering

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JoVE Journal Bioengineering
In vitro Montering af Semi-kunstige Molekylær Machine og dens anvendelse til påvisning af DNA-skader
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In vitro Montering af Semi-kunstige Molekylær Machine og dens anvendelse til påvisning af DNA-skader

Article DOI: 10.3791/3628-v • 08:56 min • January 11th, 2012
January 11th, 2012 •
1,2, 1,2,3
1Neurosurgery, Baylor College of Medicine, 2Michael E. DeBakey Veterans Affairs Medical Center, 3Molecular & Cellular Biology, Baylor College of Medicine

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Vi viser monteringen og anvendelsen af ​​en molekylær-skala-enhed drevet af en topoisomerase protein. Det begreb er en bio-molekylære sensor, som etiketter to store typer af DNA-brud i vævssnit ved at knytte to forskellige fluorophores til deres mål.

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Bioteknik molekylær maskine bio-nanoteknologi 5'OH DNA pauser 5'PO4 DNA pauser apoptose mærkning in situ detektion vaccinia topoisomerase I DNA pauser grøn nanoteknologi
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