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JoVE Journal Bioengineering
DNA-tøjret RNA Polymerase til programmerbar In vitro transskription og molekylær beregning
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DNA-tøjret RNA Polymerase til programmerbar In vitro transskription og molekylær beregning

Article DOI: 10.3791/62073-v • 09:26 min • December 29th, 2021
December 29th, 2021 •
1, 1, 1
1Institute of Biomedical Engineering, University of Toronto

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Vi beskriver ingeniørien af en ny DNA-tøjret T7 RNA polymerase til regulering af in vitro transskription reaktioner. Vi diskuterer trinene til proteinsyntese og karakterisering, validere proof-of-concept transskriptionel regulering, og diskutere dens anvendelser i molekylær computing, diagnostik, og molekylær informationsbehandling.

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Bioengineering Udgave 178 dynamisk DNA nanoteknologi molekylær programmering molekylær databehandling in vitro-genkredsløb in vitro transskription toehold-medieret strengforskydning
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