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JoVE Journal Cancer Research
Four-Color Fluorescence Immunohistochemistry af T-Cell Subpopulations i Archival Formalin-Fixed, Paraffin-Embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
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Four-Color Fluorescence Immunohistochemistry af T-Cell Subpopulations i Archival Formalin-Fixed, Paraffin-Embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Article DOI: 10.3791/55589-v • 07:43 min • July 29th, 2017
July 29th, 2017 •
1, 2, 1,3
1Department of Pathology, Leiden University Medical Center, 2Department of Otorhinolaryngology and Head and Neck Surgery, Erasmus University Medical Center, 3Center for Gynecological Oncology Amsterdam, VU Medical Center

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Multiparameterfluorescensimmunohistokemi kan anvendes til at vurdere antallet, den relative fordeling og lokalisering af immuncellepopulationer i tumormikromiljøet. Dette manuskript beskriver brugen af ​​denne teknik til analyse af T-celle subpopulationer i orofaryngealkræft.

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Cancer Research Immunology head and neck cancer tumor microenvironment fluorescence microscopy T celle Th17 celle Treg IL-17
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