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Journal / Developmental Biology / Generatie van genetisch gemodificeerde muizen via …
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JoVE Journal Developmental Biology
Generatie van genetisch gemodificeerde muizen via de microinjectie van oocyten
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Generatie van genetisch gemodificeerde muizen via de microinjectie van oocyten

Article DOI: 10.3791/55765-v • 10:19 min • June 15th, 2017
June 15th, 2017 •
1, 1
1Transgenic Animal Unit, Mark Wainwright Analytical Centre, University of New South Wales

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De microinjectie van muis oocyten wordt vaak gebruikt voor zowel klassieke transgenese ( dwz de willekeurige integratie van transgenen) en CRISPR-gemedieerde gen targeting. Dit protocol beoordeelt de nieuwste ontwikkelingen in microinjectie, met speciale nadruk op kwaliteitscontrole en genotyping strategieën.

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Ontwikkelingsbiologie Uitgave 124 Muizen Microinjectie pronucleair cytoplasmatisch CRISPR Transgenic Genoom Editing Oocyten
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