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Developmental Biology

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JoVE Journal Developmental Biology
Zygote micro-injectie voor het maken van gencassette knock-in en flox-allelen bij muizen
Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles
Journal (Developmental Biology)
Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles
Automating iPSC Culture for Enhanced Reproducibility (Video) | JoVE
Journal (Developmental Biology)
Automating iPSC Culture for Enhanced Reproducibility (Video) | JoVE
 
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Zygote micro-injectie voor het maken van gencassette knock-in en flox-allelen bij muizen

Article DOI: 10.3791/64161-v • 08:48 min • June 24th, 2022
June 24th, 2022 •
*1, *1,2, 1, 1, 1, 1, 1, 1
1Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba
* These authors contributed equally

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Het huidige protocol beschrijft zygote micro-injectie van CRISPR-Cas9 en donor-DNA om efficiënt gencassette knock-in en floxed muizen te produceren.

Tags

Developmental Biology CRISPR-Cas9 genome editing genetically modified mouse gene cassette knock-in flox zygote microinjection piezo
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