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In Vitro Saggio di SUMOilazione per studiare l'attività E3 ligasi SUMO
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In Vitro Saggio di SUMOilazione per studiare l'attività E3 ligasi SUMO

Article DOI: 10.3791/56629-v • 09:45 min • January 29th, 2018
January 29th, 2018 •
1, 2, 2,3,4,5, 1,6
1Institute of Microbiology and Immunology, National Yang-Ming University, 2UC Davis Cancer Center, University of California, Davis, 3Department of Biochemistry and Molecular Medicine, University of California, Davis, 4Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 5Division of Molecular and Genomic Medicine, National Health Research Institutes, 6Center for Infectious Disease and Cancer Research, Kaohsiung Medical University

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A differenza di ubiquitin-ligasi, sono stati identificati alcuni ligasi E3 SUMO. Questo modificate in vitro sumoilazione protocollo è in grado di identificare il romanzo SUMO E3 ligasi mediante saggio di ricostituzione in vitro .

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Biologia molecolare problema 131 sumoilazione In vitro K-bZIP SUMO E3 ligasi p53 modificazione post-traduzionale Ubc9
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