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In Vitro SUMOylation analysen å studere SUMO E3 Ligase aktivitet
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In Vitro SUMOylation analysen å studere SUMO E3 Ligase aktivitet

Article DOI: 10.3791/56629-v • 09:45 min • January 29th, 2018
January 29th, 2018 •
1, 2, 2,3,4,5, 1,6
1Institute of Microbiology and Immunology, National Yang-Ming University, 2UC Davis Cancer Center, University of California, Davis, 3Department of Biochemistry and Molecular Medicine, University of California, Davis, 4Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 5Division of Molecular and Genomic Medicine, National Health Research Institutes, 6Center for Infectious Disease and Cancer Research, Kaohsiung Medical University

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I motsetning til ubiquitin ligases, har noen E3 SUMO ligases blitt identifisert. Dette endret i vitro SUMOylation protokollen er kjøpedyktig identifisere romanen SUMO E3 ligases ved i vitro rekonstituering analysen.

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Molekylærbiologi problemet 131 i vitro SUMOylation K-bZIP SUMO E3 ligase p53 post-translasjonell modifikasjon Ubc9
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