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Journal / Biology / In Vitro SUMOylation Assay att studera SUMO E3…
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JoVE Journal Biology
In Vitro SUMOylation Assay att studera SUMO E3 Ligase aktivitet
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In Vitro SUMOylation Assay att studera SUMO E3 Ligase aktivitet

Article DOI: 10.3791/56629-v • 09:45 min • January 29th, 2018
January 29th, 2018 •
1, 2, 2,3,4,5, 1,6
1Institute of Microbiology and Immunology, National Yang-Ming University, 2UC Davis Cancer Center, University of California, Davis, 3Department of Biochemistry and Molecular Medicine, University of California, Davis, 4Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 5Division of Molecular and Genomic Medicine, National Health Research Institutes, 6Center for Infectious Disease and Cancer Research, Kaohsiung Medical University

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Till skillnad från ubiquitin ligases, har några E3 SUMO ligases identifierats. Denna modifierade in vitro- SUMOylation protokoll är kunna identifiera nya SUMO E3 ligases genom beredning in vitro- test.

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Molekylärbiologi fråga 131 In vitro SUMOylation K-bZIP SUMO E3 ligase p53 post-translationella modifieringar Ubc9
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