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Journal / Genetics / Identificatie van voetafdrukken van RNA:…
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JoVE Journal Genetics
Identificatie van voetafdrukken van RNA
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Identificatie van voetafdrukken van RNA: eiwitcomplexen via RNA-immunoprecipitatie in tandem gevolgd door sequencing (RIPiT-SEQ)

Article DOI: 10.3791/59913-v • 09:26 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1
1Department of Molecular Genetics, Center for RNA Biology, The Ohio State University

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Hier presenteren we een protocol om endogene RNA binding sites of "Footprints" van RNA: proteïne (RNP) complexen uit zoogdiercellen te verrijken. Deze aanpak omvat twee immunoprecipitaties van RNP-subeenheden en wordt daarom nagesynchroniseerde RNA-immunoprecipitatie in tandem (RIPiT).

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Genetica RNA footprint RIPiT RNA-bindend proteïne RBP RNA: proteïne complexen RNP
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