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JoVE Journal Genetics
Identificazione delle impronte di RNA
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Identificazione delle impronte di RNA:Complessi proteici tramite l'immunoprecipitazioni dell'RNA in Tandem seguita dal sequenziamento (RIPiT-Seq)

Article DOI: 10.3791/59913-v • 09:26 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1
1Department of Molecular Genetics, Center for RNA Biology, The Ohio State University

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Qui, presentiamo un protocollo per arricchire i siti endogeni di legame dell'RNA o "impronte" di nasi: proteine (RNP) complessi da cellule di mammiferi. Questo approccio comporta due immunoprecipitazioni di sottounità RNP ed è quindi soprannominato immunoprecipitazioni di RNA in tandem (RIPiT).

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Genetica Numero 149 Impronta RNA RIPiT Proteina RNA-legante RBP complessi RNA:proteine RNP
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