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Journal / Genetics / Identifisering av fotavtrykk av RNA: protein…
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Genetics

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JoVE Journal Genetics
Identifisering av fotavtrykk av RNA
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Identifisering av fotavtrykk av RNA: protein komplekser via RNA-Immunutfelling i tandem etterfulgt av sekvensering (RIPiT-SEQ)

Article DOI: 10.3791/59913-v • 09:26 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1
1Department of Molecular Genetics, Center for RNA Biology, The Ohio State University

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Her presenterer vi en protokoll for å berike endogene RNA bindende nettsteder eller "fotavtrykk" av RNA: protein (RNP) komplekser fra pattedyrceller. Denne tilnærmingen innebærer to immunoprecipitations av RNP under enheter og er derfor kalt RNA immunutfelling i tandem (RIPiT).

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Genetikk RNA fotavtrykk RIPiT RNA-bindende protein RBP RNA: protein komplekser RNP
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