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Journal / Immunology and Infection / Fordøjelse af hele museøjne til…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Fordøjelse af hele museøjne til multiparameterflow cytometrisk analyse af mononukleare fagocytter
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Fordøjelse af hele museøjne til multiparameterflow cytometrisk analyse af mononukleare fagocytter

Article DOI: 10.3791/61348-v • 09:58 min • June 17th, 2020
June 17th, 2020 •
1, *2, *1
1Department of Ophthalmology, Feinberg School of Medicine, Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine, Northwestern University
* These authors contributed equally

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Denne protokol giver en metode til at fordøje hele øjne i en enkelt celle suspension med henblik på multi-parameter flow cytometrisk analyse med henblik på at identificere specifikke okulære mononukleare phagocytiske populationer, herunder monocytter, mikroglia, makrofager, og dendritiske celler.

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Immunologi og infektion Problem 160 øje flow cytometry makrofag microglia monocyt myeloid
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