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Agrobacterium-medieretgenetisk transformation, transgene produktion, og dens anvendelse til undersøgelse af mandlige reproduktive udvikling i ris
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Agrobacterium-medieretgenetisk transformation, transgene produktion, og dens anvendelse til undersøgelse af mandlige reproduktive udvikling i ris

Article DOI: 10.3791/61665-v • 07:43 min • October 6th, 2020
October 6th, 2020 •
*1,2, *1, 1, 3, 1,3
1Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 2College of Agronomy, Anhui Agricultural University, Heifei, China, 3School of Agriculture, Food and Wine, University of Adelaide
* These authors contributed equally

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Dette arbejde beskriver brugen af CRISPR-Cas9 genom redigering teknologi til knockout endogene gen OsABCG15 efterfulgt af en modificeret Agrobacterium-medierettransformation protokol til at producere en stabil mand-steril linje i ris.

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Denne måned i JoVE ris CRISPR-Cas9 Agrobacterium callus væv kultur genotype fænotype anther pollen
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