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Agrobacterium-Mediert genetisk transformasjon, transgen produksjon, og dens anvendelse for studiet av mannlig reproduktiv utvikling i ris
Preparation of Quality Inositol Pyrophosphates
Journal (Biology)
Preparation of Quality Inositol Pyrophosphates
Mitochondrial Distribution Analysis Using Fast Fourier Transform (Video) | JoVE
Journal (Biology)
Mitochondrial Distribution Analysis Using Fast Fourier Transform (Video) | JoVE
 
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Agrobacterium-Mediert genetisk transformasjon, transgen produksjon, og dens anvendelse for studiet av mannlig reproduktiv utvikling i ris

Article DOI: 10.3791/61665-v • 07:43 min • October 6th, 2020
October 6th, 2020 •
*1,2, *1, 1, 3, 1,3
1Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 2College of Agronomy, Anhui Agricultural University, Heifei, China, 3School of Agriculture, Food and Wine, University of Adelaide
* These authors contributed equally

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Dette arbeidet beskriver bruken av CRISPR-Cas9 genomredigeringsteknologi for å slå ned endogene genet OsABCG15 etterfulgt av en modifisert Agrobacterium-medierttransformasjonsprotokoll for å produsere en stabil mannssteril linje i ris.

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Denne måneden i JoVE ris CRISPR-Cas9 Agrobacterium callus vev kultur genotype fenotype anther pollen
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