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Neuroscience

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JoVE Journal Neuroscience
Langsigtet, Serumfri Dyrkning af organotypiske musehindeplanter med intakt retinale pigmentepitelet
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Langsigtet, Serumfri Dyrkning af organotypiske musehindeplanter med intakt retinale pigmentepitelet

Article DOI: 10.3791/61868-v • 05:52 min • November 25th, 2020
November 25th, 2020 •
*1, *1, 1, 1, 1, 1
1Institute for Ophthalmic Research, University of Tübingen
* These authors contributed equally

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Protokollen beskriver organotypiske explants af mus neuronethinden, dyrket sammen med sin nethinde pigment epitel (RPE), i R16 defineret medium, fri for serum og antibiotika. Denne metode er relativt enkel at udføre, billigere og tidskrævende sammenlignet med in vivo-eksperimenter og kan tilpasses til adskillige eksperimentelle applikationer.

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Neurovidenskab neuroretina RPE organotypiske explant kulturer serum-fri medium mus organkultur
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