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Neuroscience

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JoVE Journal Neuroscience
Cultivo de longo prazo, sem soro de explantes de retina de rato organotipo com epitélio pigmento de retina intacto
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Cultivo de longo prazo, sem soro de explantes de retina de rato organotipo com epitélio pigmento de retina intacto

Article DOI: 10.3791/61868-v • 05:52 min • November 25th, 2020
November 25th, 2020 •
*1, *1, 1, 1, 1, 1
1Institute for Ophthalmic Research, University of Tübingen
* These authors contributed equally

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O protocolo descreve explanações organotípicas daretina neurodo camundongo, cultivadas juntamente com seu epitélio pigmento retiniano (RPE), em meio definido r16, livre de soro e antibióticos. Este método é relativamente simples de executar, menos caro e demorado quando comparado com experimentos in vivo, e pode ser adaptado a inúmeras aplicações experimentais.

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Neurociência Edição 165 neuroretina RPE culturas organotípicas de explant meio sem soro rato cultura de órgãos
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