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Journal / Neuroscience / Langsiktig, serumfri dyrking av organotypisk mus…
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Neuroscience

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JoVE Journal Neuroscience
Langsiktig, serumfri dyrking av organotypisk mus retina explants med intakt retinal pigment epitel
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Langsiktig, serumfri dyrking av organotypisk mus retina explants med intakt retinal pigment epitel

Article DOI: 10.3791/61868-v • 05:52 min • November 25th, 2020
November 25th, 2020 •
*1, *1, 1, 1, 1, 1
1Institute for Ophthalmic Research, University of Tübingen
* These authors contributed equally

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Protokollen beskriver organotypiske utvisning av mus neuronetthinne, dyrket sammen med sin retinal pigment epitel (RPE), i R16 definert medium, fri for serum og antibiotika. Denne metoden er relativt enkel å utføre, billigere og tidkrevende sammenlignet med in vivo-eksperimenter, og kan tilpasses mange eksperimentelle applikasjoner.

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Nevrovitenskap Utgave 165 neuroretina RPE organotypiske utvisningskulturer serumfritt medium mus organkultur
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