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JoVE Journal Behavior
Vergelijkende analyse van experimentele methoden om dierlijke activiteit in Caenorhabditis elegans-modellen van mitochondriale ziekte te kwantificeren
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Vergelijkende analyse van experimentele methoden om dierlijke activiteit in Caenorhabditis elegans-modellen van mitochondriale ziekte te kwantificeren

Article DOI: 10.3791/62244-v • 05:51 min • April 4th, 2021
April 4th, 2021 •
*1, *1, 1, 1,2, 1,2
1Mitochondrial Medicine Frontier Program, Division of Human Genetics, Department of Pediatrics, The Children’s Hospital of Philadelphia, 2Department of Pediatrics, University of Pennsylvania Perelman School of Medicine
* These authors contributed equally

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Deze studie presenteert protocollen voor twee semi-geautomatiseerde locomotorische activiteitsanalysebenaderingen in C. elegans complex I ziekte gas-1(fc21) wormen, namelijk ZebraLab (een medium-throughput assay) en WormScan (een high-throughput assay) en biedt vergelijkende analyse tussen een breed scala aan onderzoeksmethoden om het gedrag van nematoden en de geïntegreerde neuromusculaire functie te kwantificeren.

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Gedrag wormen C. elegans locomotorische activiteit faryngeaal pompen chemotaxis thrashing ZebraLab WormScan high-throughput screening capaciteit gas-1 (fc21)
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