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Behavior

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JoVE Journal Behavior
Komparativ analyse av eksperimentelle metoder for å kvantifisere dyreaktivitet i Caenorhabditis elegans Modeller av mitokondriesykdom
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Komparativ analyse av eksperimentelle metoder for å kvantifisere dyreaktivitet i Caenorhabditis elegans Modeller av mitokondriesykdom

Article DOI: 10.3791/62244-v • 05:51 min • April 4th, 2021
April 4th, 2021 •
*1, *1, 1, 1,2, 1,2
1Mitochondrial Medicine Frontier Program, Division of Human Genetics, Department of Pediatrics, The Children’s Hospital of Philadelphia, 2Department of Pediatrics, University of Pennsylvania Perelman School of Medicine
* These authors contributed equally

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Denne studien presenterer protokoller for to halvautomatiske lokomotoriske aktivitetsanalyser i C. elegans kompleks I sykdom gass-1(fc21) ormer, nemlig ZebraLab (en middels gjennomstrømningsanalyse) og WormScan (en høy gjennomstrømningsanalyse) og gir komparativ analyse blant et bredt spekter av forskningsmetoder for å kvantifisere nematodeadferd og integrert nevromuskulær funksjon.

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Oppførsel utgave 170 ormer C. elegans lokomotorisk aktivitet pharyngeal pumping chemotaxis thrashing ZebraLab WormScan høy gjennomstrømning screening kapasitet gass-1 (fc21)
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