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Journal / Biochemistry / Kontinuerlig fluorescensbaseret…
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Biochemistry

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JoVE Journal Biochemistry
Kontinuerlig fluorescensbaseret endonukleasekoblet DNA-methyleringsassay til screening for DNA-methyltransferasehæmmere
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Kontinuerlig fluorescensbaseret endonukleasekoblet DNA-methyleringsassay til screening for DNA-methyltransferasehæmmere

Article DOI: 10.3791/62949-v • 06:07 min • August 5th, 2022
August 5th, 2022 •
1, 2, 2
1Department of Chemistry, Bucknell University, 2Program in Cell Biology/Biochemistry, Bucknell University

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DNA-methyltransferaser er potentielle kræftlægemiddelmål. Her præsenteres en protokol til vurdering af små molekyler til DNA-methyltransferasehæmning. Dette assay anvender en endonuklease til at koble DNA-methylering til fluorescensgenerering og gør det muligt at overvåge enzymaktivitet i realtid.

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Biokemi udgave 186 biokemi udgave ,
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