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Journal / Biochemistry / Ensayo continuo de metilación del ADN acoplado a…
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Biochemistry

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JoVE Journal Biochemistry
Ensayo continuo de metilación del ADN acoplado a endonucleasa basado en fluorescencia para detectar inhibidores de la metiltransferasa de ADN
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Ensayo continuo de metilación del ADN acoplado a endonucleasa basado en fluorescencia para detectar inhibidores de la metiltransferasa de ADN

Article DOI: 10.3791/62949-v • 06:07 min • August 5th, 2022
August 5th, 2022 •
1, 2, 2
1Department of Chemistry, Bucknell University, 2Program in Cell Biology/Biochemistry, Bucknell University

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Las metiltransferasas de ADN son posibles objetivos farmacológicos contra el cáncer. Aquí, se presenta un protocolo para evaluar moléculas pequeñas para la inhibición de la metiltransferasa de ADN. Este ensayo utiliza una endonucleasa para acoplar la metilación del ADN a la generación de fluorescencia y permite monitorear la actividad enzimática en tiempo real.

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Bioquímica Número 186 Bioquímica Número ,
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