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Journal / Biochemistry / Dual-Color Fluorescens Krydskorrelation…
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Biochemistry
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JoVE Journal Biochemistry
Dual-Color Fluorescens Krydskorrelation Spektroskopi til undersøgelse protein-protein interaktion og protein dynamik i levende celler
Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle
Journal (Biochemistry)
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A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
Journal (Biochemistry)
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Dual-Color Fluorescens Krydskorrelation Spektroskopi til undersøgelse protein-protein interaktion og protein dynamik i levende celler

Article DOI: 10.3791/62954-v • 14:12 min • December 11th, 2021
December 11th, 2021 •
*1, *1, 1, 1, 1, 2, 1
1Rudolf Virchow Center for Integrative and Translation Bioimaging, Julius-Maximilian University Wuerzburg, 2Max Delbrück Center for Molecular Medicine, Berlin
* These authors contributed equally

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Vi præsenterer en eksperimentel protokol og dataanalyse workflow til at udføre levende celle dual-color fluorescens kryds korrelation spektroskopi (FCCS) kombineret med Förster Resonans Energioverførsel (FRET) til at studere membran receptor dynamik i levende celler ved hjælp af moderne fluorescens mærkning teknikker.

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