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Journal / Biochemistry / Fluorescens spektroskopi med dubbla färger för…
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Biochemistry
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JoVE Journal Biochemistry
Fluorescens spektroskopi med dubbla färger för att studera protein-proteininteraktion och proteindynamik i levande celler
An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
Journal (Biochemistry)
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A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro
Journal (Biochemistry)
A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro
 
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Fluorescens spektroskopi med dubbla färger för att studera protein-proteininteraktion och proteindynamik i levande celler

Article DOI: 10.3791/62954-v • 14:12 min • December 11th, 2021
December 11th, 2021 •
*1, *1, 1, 1, 1, 2, 1
1Rudolf Virchow Center for Integrative and Translation Bioimaging, Julius-Maximilian University Wuerzburg, 2Max Delbrück Center for Molecular Medicine, Berlin
* These authors contributed equally

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Vi presenterar ett experimentellt protokoll och dataanalysarbetsflöde för att utföra levande cell dual-color fluorescence cross correlation spektroskopi (FCCS) kombinerat med Förster Resonance Energy transfer (FRET) för att studera membranreceptordynamik i levande celler med hjälp av moderna fluorescensmärkningstekniker.

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Biokemi nummer 178
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