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Journal / Biochemistry / Dual-Color Fluorescence Cross-Correlation…
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Biochemistry
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JoVE Journal Biochemistry
Dual-Color Fluorescence Cross-Correlation Spectroscopy om eiwit-eiwitinteractie en eiwitdynamica in levende cellen te bestuderen
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Dual-Color Fluorescence Cross-Correlation Spectroscopy om eiwit-eiwitinteractie en eiwitdynamica in levende cellen te bestuderen

Article DOI: 10.3791/62954-v • 14:12 min • December 11th, 2021
December 11th, 2021 •
*1, *1, 1, 1, 1, 2, 1
1Rudolf Virchow Center for Integrative and Translation Bioimaging, Julius-Maximilian University Wuerzburg, 2Max Delbrück Center for Molecular Medicine, Berlin
* These authors contributed equally

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We presenteren een experimenteel protocol en data-analyseworkflow om live cell dual-color fluorescentie cross correlation spectroscopie (FCCS) uit te voeren in combinatie met Förster Resonance Energy transfer (FRET) om membraanreceptordynamica in levende cellen te bestuderen met behulp van moderne fluorescentielabeltechnieken.

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Biochemie Nummer 178
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