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Journal / Biochemistry / Immunopeptidomics: isolatie van muis en humane…
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Biochemistry

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JoVE Journal Biochemistry
Immunopeptidomics
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Immunopeptidomics: isolatie van muis en humane MHC klasse I- en II-geassocieerde peptiden voor massaspectrometrie-analyse

Article DOI: 10.3791/63052-v • 09:32 min • October 15th, 2021
October 15th, 2021 •
*1, *1, 1, 1, 1,2
1CHU Sainte-Justine Research Center, 2Department of Pathology and Cellular Biology, Faculty of Medicine, Université de Montréal
* These authors contributed equally

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Hier presenteren we een protocol voor de zuivering van MHC klasse I en klasse II peptidecomplexen uit muis- en menselijke cellijnen die hoogwaardige immunopeptidomics-gegevens leveren. Het protocol richt zich op monstervoorbereiding met behulp van in de handel verkrijgbare antilichamen.

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Biochemie Nummer 176
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