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Journal / Biochemistry / Immunopeptidomics: Isolamento di peptidi…
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Biochemistry

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JoVE Journal Biochemistry
Immunopeptidomics
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Immunopeptidomics: Isolamento di peptidi associati A MHC di classe I e II di topi e umani per l'analisi della spettrometria di massa

Article DOI: 10.3791/63052-v • 09:32 min • October 15th, 2021
October 15th, 2021 •
*1, *1, 1, 1, 1,2
1CHU Sainte-Justine Research Center, 2Department of Pathology and Cellular Biology, Faculty of Medicine, Université de Montréal
* These authors contributed equally

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Qui, presentiamo un protocollo per la purificazione di complessi peptidici MHC di classe I e classe II da linee cellulari di topi e umani che forniscono dati immunopeptidomici di alta qualità. Il protocollo si concentra sulla preparazione del campione utilizzando anticorpi disponibili in commercio.

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Biochimica Numero 176
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