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Journal / Biochemistry / Imunopeptidomics: Isolamento de Peptídeos MHS de…
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Biochemistry

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JoVE Journal Biochemistry
Imunopeptidomics
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Imunopeptidomics: Isolamento de Peptídeos MHS de classe I e II associados para análise de espectrometria de massa

Article DOI: 10.3791/63052-v • 09:32 min • October 15th, 2021
October 15th, 2021 •
*1, *1, 1, 1, 1,2
1CHU Sainte-Justine Research Center, 2Department of Pathology and Cellular Biology, Faculty of Medicine, Université de Montréal
* These authors contributed equally

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Aqui, apresentamos um protocolo para a purificação dos complexos de peptídeos classe I e CLASSE II da classe I, fornecendo dados de imunopeptidomias de alta qualidade. O protocolo se concentra na preparação da amostra usando anticorpos disponíveis comercialmente.

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Bioquímica Edição 176
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