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Biochemistry

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JoVE Journal Biochemistry
Shotgun Proteomics Sample Processing geautomatiseerd door een open-source labrobot
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Shotgun Proteomics Sample Processing geautomatiseerd door een open-source labrobot

Article DOI: 10.3791/63092-v • 10:12 min • October 28th, 2021
October 28th, 2021 •
1,3, 1,3, 1,3, 1,3, 1,2,3
1Department of Medicine-Cardiology, University of Colorado Anschutz Medical Campus, 2Department of Biochemistry & Molecular Genetics, University of Colorado Anschutz Medical Campus, 3Consortium for Fibrosis Research & Translation, School of Medicine, University of Colorado Anschutz Medical Campus

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Gedetailleerd protocol en drie Python-scripts zijn voorzien voor het bedienen van een open-source robotisch vloeistofverwerkingssysteem om semi-geautomatiseerde eiwitmonstervoorbereiding uit te voeren voor massaspectrometrie-experimenten, met betrekking tot wasmiddelverwijdering, eiwitvertering en peptide-ontzoutingsstappen.

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Biochemie Nummer 176
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