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Isolamento de células endoteliais do lúmen de artérias carótidas de camundongos para experimentos multi-ômicos unicelulares
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Isolamento de células endoteliais do lúmen de artérias carótidas de camundongos para experimentos multi-ômicos unicelulares

Article DOI: 10.3791/63128-v • 09:12 min • October 4th, 2021
October 4th, 2021 •
*1, *1, 1, 1, 1, 1,2
1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 2Division of Cardiology, Georgia Institute of Technology and Emory University
* These authors contributed equally

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Apresentamos um método para isolar células e núcleos endoteliais do lúmen de artérias carótidas de camundongos expostas a condições de fluxo estáveis ou perturbadas para a realização de experimentos ômicos unicelulares.

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Biologia Edição 176 Células arteriais Células endoteliais RNAseq de célula única ATACseq de célula única Aterosclerose
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