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JoVE Journal Genetics
Bestemme rollen til maternalt uttrykte gener i tidlig utvikling med mors crispants
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Bestemme rollen til maternalt uttrykte gener i tidlig utvikling med mors crispants

Article DOI: 10.3791/63177-v • 10:08 min • December 21st, 2021
December 21st, 2021 •
1, 1, 1
1Laboratory of Genetics, University of Wisconsin-Madison

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Tidlig utvikling er avhengig av mors arvede produkter, og rollen til mange av disse produktene er foreløpig ukjent. Her beskrev vi en protokoll som bruker CRISPR-Cas9 for å identifisere fenotyper med morseffekt i en enkelt generasjon.

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Genetikk utgave 178 tidlig utvikling CRISPR-Cas9 morseffekt sebrafisk genomredigering omvendt genetikk
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