Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / In vitro Karakterisering af histon chaperoner ved …
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biochemistry
In vitro Karakterisering af histon chaperoner ved hjælp af analytiske, pull-down og chaperoning assays
Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain
Journal (Biochemistry)
Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
Journal (Biochemistry)
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
 
Click here for the English version

In vitro Karakterisering af histon chaperoner ved hjælp af analytiske, pull-down og chaperoning assays

Article DOI: 10.3791/63218-v • 08:16 min • December 29th, 2021
December 29th, 2021 •
*1,2, *1,2, 1,3, 1,2, 1,2, 1, 1,4, 1,5, 1, 1
1Institute of Life Sciences, 2Regional Centre for Biotechnology, 3School of Biotechnology, KIIT University, 4Department of Molecular Biophysics and Biochemistry, Yale University, 5Department of Pharmacology, University of Vermont College of Medicine
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol beskriver et batteri af metoder, der inkluderer analytisk størrelsesekskluderingskromatografi til at studere histon chaperone oligomerisering og stabilitet, pull-down assay for at opklare histon chaperon-histon-interaktioner, AUC til at analysere støkiometrien af proteinkomplekserne og histon chaperoning assay for funktionelt at karakterisere en formodet histon chaperone in vitro.

Tags

Biokemi udgave 178 histon chaperon nukleosomsamling kromatin nukleoplasmin NAP plasmid supercoiling analytisk ultracentrifugering
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter