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Journal / Biochemistry / Rensing av endogene Drosophila transiente…
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JoVE Journal Biochemistry
Rensing av endogene Drosophila transiente reseptorpotensialkanaler
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Rensing av endogene Drosophila transiente reseptorpotensialkanaler

Article DOI: 10.3791/63260-v • 08:39 min • December 28th, 2021
December 28th, 2021 •
*1, *1, 1, 1, 1, 1
1Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center
* These authors contributed equally

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Basert på monteringsmekanismen til INAD-proteinkomplekset, ble det i denne protokollen utviklet en modifisert affinitetsrensing pluss konkurransestrategi for å rense den endogene Drosophila TRP-kanalen.

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Biokjemi utgave 178 proteinrensing Drosophila TRP-kanal INAD
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