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Journal / Medicine / 小鼠后肢坏死模型中脚垫脉管系统的高分辨率三维成像…
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小鼠后肢坏死模型中脚垫脉管系统的高分辨率三维成像

Article DOI: 10.3791/63284-v • 08:16 min • March 16th, 2022
March 16th, 2022 •
1, 1,2, 3, 1, 1,2, 4, 1,2, 1,2, 1,2
1Dewitt Daughtry Family Department of Surgery, University of Miami Miller School of Medicine, 2Vascular Biology Institute, University of Miami Miller School of Medicine, 3University of Miami, 4Analytical Imaging Core Facility, University of Miami

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本方案描述了一种独特的、临床相关的外周动脉疾病模型，该模型将股动脉和静脉电凝与施用一氧化氮合酶抑制剂相结合，以诱导FVB小鼠的后肢坏疽。然后，心内DiI灌注用于脚垫脉管系统的高分辨率三维成像。

Tags

医学，第181期，后肢缺血，坏疽，股动脉结扎，L-NAME，DiI灌注，外周动脉疾病，新生血管形成，血管生成
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