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JoVE Journal Medicine
Høyoppløselig tredimensjonal bildebehandling av footpad vaskulaturen i en Murine hindlimb gangrene modell
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Høyoppløselig tredimensjonal bildebehandling av footpad vaskulaturen i en Murine hindlimb gangrene modell

Article DOI: 10.3791/63284-v • 08:16 min • March 16th, 2022
March 16th, 2022 •
1, 1,2, 3, 1, 1,2, 4, 1,2, 1,2, 1,2
1Dewitt Daughtry Family Department of Surgery, University of Miami Miller School of Medicine, 2Vascular Biology Institute, University of Miami Miller School of Medicine, 3University of Miami, 4Analytical Imaging Core Facility, University of Miami

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Den nåværende protokollen beskriver en unik, klinisk relevant modell av perifer arteriell sykdom som kombinerer lårarterie og veneelektrokoagulasjon med administrering av en nitrogenoksidsyntasehemmer for å indusere hindlimb gangren hos FVB-mus. Intracardiac DiI perfusjon brukes deretter til høyoppløselig, tredimensjonal bildebehandling av fotputens vaskulatur.

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Medisin Utgave 181 Hindlimb iskemi koldbrann lårarterie ligation L-NAME DiI perfusjon perifer arteriell sykdom neovascularization angiogenese
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