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Productie van High-titer Recombinant Newcastle Disease Virus uit Allantoic Fluid
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Productie van High-titer Recombinant Newcastle Disease Virus uit Allantoic Fluid

Article DOI: 10.3791/63817-v • 07:24 min • May 25th, 2022
May 25th, 2022 •
1, 1, 1, 1, 1, 1, 1
1Department of Pathobiology, University of Guelph

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Hier bieden we een gedetailleerde procedure voor de productie, zuivering en kwantificering van high-titer recombinant Newcastle disease virus. Dit protocol levert consequent > 6 × 109 plaquevormende eenheden/ml op, wat virushoeveelheden oplevert die geschikt zijn voor in vivo dierstudies. Aanvullende kwaliteitscontroletests om de veiligheid in vivo te waarborgen, worden beschreven.

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Kankeronderzoek Nummer 183
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