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JoVE Journal Cancer Research
Produksjon av high-titer rekombinant Newcastle Disease Virus fra Allantoic Fluid
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Produksjon av high-titer rekombinant Newcastle Disease Virus fra Allantoic Fluid

Article DOI: 10.3791/63817-v • 07:24 min • May 25th, 2022
May 25th, 2022 •
1, 1, 1, 1, 1, 1, 1
1Department of Pathobiology, University of Guelph

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Her gir vi en detaljert prosedyre for produksjon, rensing og kvantifisering av høy-titer rekombinant Newcastle disease virus. Denne protokollen gir konsekvent > 6 × 109 plakkdannende enheter/ml, og gir virusmengder som er egnet for in vivo dyrestudier. Ytterligere kvalitetskontrollanalyser for å sikre sikkerhet in vivo er beskrevet.

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