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JoVE Journal Cancer Research
Fremstilling af cytoplasmatiske og nukleare lange RNA'er fra primære og dyrkede celler
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Fremstilling af cytoplasmatiske og nukleare lange RNA'er fra primære og dyrkede celler

Article DOI: 10.3791/64199-v • 06:29 min • April 7th, 2023
April 7th, 2023 •
1, 1, 1, 1, 1, 1
1Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine

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Denne protokol tilbyder en effektiv og fleksibel metode til at isolere RNA fra nukleare og cytoplasmatiske fraktioner ved hjælp af dyrkede celler og derefter validere ved hjælp af qPCR. Dette tjener effektivt som erstatning for andre RNA-præparationssæt.

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Kræftforskning nr. 194
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