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JoVE Journal Cancer Research
Préparation d’ARN longs cytoplasmiques et nucléaires à partir de cellules primaires et en culture
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Préparation d’ARN longs cytoplasmiques et nucléaires à partir de cellules primaires et en culture

Article DOI: 10.3791/64199-v • 06:29 min • April 7th, 2023
April 7th, 2023 •
1, 1, 1, 1, 1, 1
1Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine

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Le protocole actuel offre une méthode efficace et flexible pour isoler l’ARN des fractions nucléaires et cytoplasmiques à l’aide de cellules en culture, puis valider à l’aide de la qPCR. Cela sert efficacement de remplacement pour d’autres kits de préparation d’ARN.

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Recherche sur le cancer numéro 194
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