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Preparazione di RNA lunghi citoplasmatici e nucleari da cellule primarie e in coltura
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Preparazione di RNA lunghi citoplasmatici e nucleari da cellule primarie e in coltura

Article DOI: 10.3791/64199-v • 06:29 min • April 7th, 2023
April 7th, 2023 •
1, 1, 1, 1, 1, 1
1Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine

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Il presente protocollo offre un metodo efficiente e flessibile per isolare l'RNA da frazioni nucleari e citoplasmatiche utilizzando cellule in coltura e quindi convalidare utilizzando qPCR. Questo serve efficacemente come sostituto per altri kit di preparazione dell'RNA.

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Ricerca sul cancro numero 194
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